                         SEQUENCE LISTING

<110>  LUPIN LIMITED
       LUPIN LIMITED
 
<120>  Novel Fusion Tag Offering Solubility to Insoluble Recombinant 
       Protein

<130>  FPAA2231PCT

<150>  689/KOL/2009
<151>  2009-05-01

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  42
<212>  DNA
<213>  artificial

<220>
<223>  Forward primer

<400>  1
ccgccggaat tccatatgca ctacaagcag cactgccctc ca                          42


<210>  2
<211>  51
<212>  DNA
<213>  artificial

<220>
<223>  Reverse primer

<400>  2
ccgccggaat tctttatcat catcatcgga tccgactggc tcccagcagt c                51


<210>  3
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  forward primer

<400>  3
ccgccgggat ccgatgatga tgataaaacg ccattaggcc cggcc                       45


<210>  4
<211>  39
<212>  DNA
<213>  artificial

<220>
<223>  Reverse primer

<400>  4
ccgccggaat tcaagcctta acggctccgc taaatgacg                              39


<210>  5
<211>  138
<212>  DNA
<213>  artificial

<220>
<223>  GM tag nucleotide sequence

<400>  5
atgcactaca agcagcactg ccctccaacc ccggaaactt cctgtgcaac ccagattatc       60

acctttgaaa gtttcaaaga gaacctgaag gactttctgc ttgtcatccc ctttgactgc      120

tgggagccag tcggatcc                                                    138


<210>  6
<211>  46
<212>  PRT
<213>  artificial

<220>
<223>  GM tag amino acid sequence

<400>  6

Met His Tyr Lys Gln His Cys Pro Pro Thr Pro Glu Thr Ser Cys Ala 
1               5                   10                  15      


Thr Gln Ile Ile Thr Phe Glu Ser Phe Lys Glu Asn Leu Lys Asp Phe 
            20                  25                  30          


Leu Leu Val Ile Pro Phe Asp Cys Trp Glu Pro Val Gly Ser 
        35                  40                  45      


<210>  7
<211>  321
<212>  DNA
<213>  artificial

<220>
<223>  SD nucleotide sequence

<400>  7
atgagcgatt ccgattcaga ctcggactcg gattccgatt ccgacagtga ttcagattct       60

gactcagatt ccgattctga ttctgattcg gattccgact ccgatagcga ctcagatagt      120

gactctgact cggacagcga ttctgatagc gactctgatt ccgatagcga tagcgattca      180

gatagcgatt ctgactcgga ttctgattcc gattctgact ctgacagcga ttccgatagc      240

gacagcgact ctgatagtga ttcagactct gattctgata gtgatagcga ttcggatagt      300

ggatccgatg atgatgataa a                                                321


<210>  8
<211>  107
<212>  PRT
<213>  artificial

<220>
<223>  SD amino acid sequence

<400>  8

Met Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser 
1               5                   10                  15      


Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser 
            20                  25                  30          


Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser 
        35                  40                  45              


Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser 
    50                  55                  60                  


Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser 
65                  70                  75                  80  


Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser Asp Ser 
                85                  90                  95      


Asp Ser Asp Ser Gly Ser Asp Asp Asp Asp Lys 
            100                 105         


<210>  9
<211>  531
<212>  DNA
<213>  artificial

<220>
<223>  Human GCHF nucleotide sequence

<400>  9
atgacgccat taggcccggc ctctagcctg ccacagagct tcttactgaa gtgcttagaa       60

caagtgcgca agatccaagg cgatggtgcc gccttacagg aaaagctgtg cgccacctac      120

aagttatgcc atccggagga gctggtgctg ttaggtcact ctttaggcat tccatgggcc      180

ccactgtctt cttgcccaag ccaggccctg caactggccg gctgtctgtc tcagctgcat      240

tctggcctgt ttctgtatca gggcttactg caagcgctgg agggcatcag cccagaatta      300

ggtccaaccc tggacacctt acaactggat gtggccgatt tcgcgacgac catttggcag      360

cagatggaag agctgggtat ggccccagcg ttacagccaa cgcagggtgc catgccggcc      420

tttgcgagcg cctttcaacg tcgtgcgggt ggtgttctgg ttgccagcca cctgcaatct      480

tttttagagg tgagctaccg cgtgttacgt catttagcgc agccgtaatg a               531


<210>  10
<211>  175
<212>  PRT
<213>  artificial

<220>
<223>  Human GCHF amino acid sequence

<400>  10

Met Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu 
            20                  25                  30          


Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
    50                  55                  60                  


Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His 
65                  70                  75                  80  


Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
            100                 105                 110         


Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala 
        115                 120                 125             


Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro 
                165                 170                 175 


<210>  11
<211>  177
<212>  DNA
<213>  artificial

<220>
<223>  His-GM tag nucleotide sequence

<400>  11
atgcatcatc atcatcatca tcatcactac aagcagcact gccctccaac cccggaaact       60

tcctgtgcaa cccagattat cacctttgaa agtttcaaag agaacctgaa ggactttctg      120

cttgtcatcc cctttgactg ctgggagcca gtccaggagg atgatgatga ttacgta         177


<210>  12
<211>  59
<212>  PRT
<213>  artificial

<220>
<223>  His-GM tag amino acid sequence

<400>  12

Met His His His His His His His His Tyr Lys Gln His Cys Pro Pro 
1               5                   10                  15      


Thr Pro Glu Thr Ser Cys Ala Thr Gln Ile Ile Thr Phe Glu Ser Phe 
            20                  25                  30          


Lys Glu Asn Leu Lys Asp Phe Leu Leu Val Ile Pro Phe Asp Cys Trp 
        35                  40                  45              


Glu Pro Val Gln Glu Asp Asp Asp Asp Tyr Val 
    50                  55                  


